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Insulin processing and action in adipocytes: evidence for generation of insulin‐containing vesicles by leupeptin and monensin
Author(s) -
Jeffrey Ian W.,
Flint David J.,
Ver Richard G.,
Mayer R.John
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80352-5
Subject(s) - monensin , vesicle , insulin , leupeptin , medicine , chemistry , endocrinology , incubation , golgi apparatus , pyruvate carboxylase , insulin receptor , biochemistry , biology , enzyme , membrane , cell , protease , insulin resistance
Incubation of adipocytes with 125 I‐insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125 I‐insulin (modal density about 1.20 ) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surface. The fractions comprising this peak contained vesicles, probably originating from the Golgi apparatus, and dit not appear to be contaminated with lysosomes, mitochondria or plasma membrane. Entrapment of insulin in these vesicles per se did not prevent the activation of glucose transport, acetyl‐CoA carboxylase or pyruvate dehydrogenase by insulin.