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‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases
Author(s) -
Meškauskas Arturas,
Ksenzenko Vladimir,
Shlyapnikov Mikhail,
Kryukov Valentin,
Čitavičius Donaldas
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80344-6
Subject(s) - restriction enzyme , cleavage (geology) , dna , saccharomyces cerevisiae , pigment , yeast , mutant , chemistry , endonuclease , biochemistry , microbiology and biotechnology , biology , gene , paleontology , organic chemistry , fracture (geology)
Protection of DNA from cleavage by restriction endonucleases Eco RI, Hind III, Bam HI, and Bg /II with red pigment, produced by ADE‐2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.