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15 N‐NMR studies of nitrogen assimilation and amino acid biosynthesis in the ectomycorrhizal fungus Cenococcum graniforme
Author(s) -
Martin Francis
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80331-8
Subject(s) - mycelium , nitrogen assimilation , biosynthesis , glutamine , arginine , fungus , glutamine synthetase , assimilation (phonology) , nitrogen , amino acid , biochemistry , nuclear magnetic resonance spectroscopy , alanine , biology , chemistry , stereochemistry , organic chemistry , botany , enzyme , linguistics , philosophy
15 N‐NMR spectroscopy was used to follow nitrogen assimilation and amino acid biosynthesis in the intact mycelium and extracts of the symbiotic fungus Cenococcum graniforme . The primary incorporation of 15 NH 4 + appears to proceed via the glutamine synthetase activity. Glutamine, γ‐aminobutyrate, alanine and arginine were the main labelled nitrogenous pools. The quenching of arginine in intact mycelium suggest that the amino acid is associated with a cellular component which restricts its rotational freedom.