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Bradykinin‐induced transient accumulation of inositol trisphosphate in neuron‐like cell line NG 108‐15 cells
Author(s) -
Yano Koh,
Higashida Haruhiro,
Hattori Hiroaki,
Nozawa Yoshinori
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80301-x
Subject(s) - inositol , phosphatidylinositol , second messenger system , inositol trisphosphate , bradykinin , inositol phosphate , diacylglycerol kinase , chemistry , intracellular , cell culture , phosphatidylinositol 4,5 bisphosphate , microbiology and biotechnology , biochemistry , biology , signal transduction , receptor , protein kinase c , genetics
Studies were undertaken to further elucidate the mechanism(s) by which bradykinin‐dependent phosphoinositide metabolism takes place in neuroblastoma × glioma hybrid NG108‐15 cells [(1984) J. Biol. Chem. 259, 10201–10207] using [ 3 H]inositol‐labelled cells. Bradykinin produced net increases in the level of [ 3 H]inositol phosphates, especially of [ 3 H]inositol trisphosphate which is formed transiently and most rapidly. The results indicate that bradykinin activates a phosphodiesterase to break down phosphatidylinositol 4,5‐bisphosphate, generating two recently recognized intracellular messengers, 1,2‐diacylglycerol and inositol trisphosphate.