Premium
Pertussis toxin facilitates the progesterone‐induced maturation of Xenopus oocyte
Author(s) -
Mulner Odile,
Megret Françoise,
Alouf Joseph E.,
Ozon René
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80300-8
Subject(s) - oocyte , xenopus , pertussis toxin , chemistry , toxin , medicine , andrology , endocrinology , microbiology and biotechnology , biology , biochemistry , g protein , receptor , embryo , gene
Progesterone triggers the first meiotic cell division of Xenopus oocyte and inhibits cAMP synthesis. The effect of pertussis toxin purified from Bordetella pertussis was tested on the maturation of Xenopus oocyte. The toxin did not inhibit progesterone‐induced resumption of meiosis or the hormone‐induced drop in cAMP level. This indicates that progesterone action is not mediated by the N i subunit of the oocyte adenylate cyclase. Furthermore, pertussis toxin caused a reduction in the time course of maturation correlated with the precocious appearance of an alkali stable 47kDa phosphoprotein, a marker of the maturation promoting factor (MPF) activity. Pertussis toxin effects mimicked those of 2‐glycerophosphate suggesting that both agents act on the steady‐state level of phosphorylation implicated in MPF activity.