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Molecular cloning and expression in Streptomyces lividans of a streptomycin 6‐phosphotransferase gene from a streptomycin‐producing microorganism
Author(s) -
Shinkawa Hidenori,
Sugiyama Masanori,
Nimi Osamu,
Nomi Ryosaku
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80298-2
Subject(s) - streptomycin , streptomyces griseus , plasmid , biology , streptomycetaceae , streptomyces , microbiology and biotechnology , gene , genetics , actinomycetales , bacteria , antibiotics
The gene encoding streptomycin 6‐kinase involved in the self‐resistance of the streptomycin‐producing Streptomyces griseus HUT 6037 was cloned in the plasmid vector pIJ703. The resulting plasmid, pSP6, contained 2.5 kb inserts of S. griseus DNA. When streptomycin‐susceptible S. lividans 1326 was retransformed with pSP6, all transformants produced streptomycin 6‐kinase. Addition of streptomycin to the culture medium of S. lividans carrying pSP6 plasmid brought about a remarkable increase in streptomycin 6‐kinase activity in the cell extracts. It is suggested from the results that the production of streptomycin 6‐kinase in streptomycin producer was induced by streptomycin accumulated during cultivation.