Premium
Tobacco plants transformed with the Agrobacterium T‐DNA gene 1 contain high amounts of indole‐3‐acetamide
Author(s) -
Van Onckelen H.,
Rüdelsheim P.,
Inzé D.,
Follin A.,
Messens E.,
Horemans S.,
Schell J.,
Van Montagu M.,
De Greef J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80295-7
Subject(s) - nicotiana tabacum , agrobacterium tumefaciens , agrobacterium , gene , biology , ti plasmid , dna , nicotiana , plasmid , transformation (genetics) , plant cell , genetics , microbiology and biotechnology , solanaceae
The T‐DNA genes 1 and 2 of the Ti plasmid of Agrobacterium tumefaciens are involved in the biosynthesis of IAA in transformed plant cells. Previously, it has been shown that gene 2 codes for an amidohydrolase able to convert IAM into IAA. We have isolated Nicotiana tabacum regenerates transformed with either gene 1 or genes 6a and 6b of the T‐DNA. The tobacco plants transformed with gene 1 contain 500–1000‐times more IAM as compared to plants transformed with genes 6a and 6b , and as compared to untransformed control plants. No drastic differences in endogenous IAA concentrations were observed between the three plant types analyzed.