Premium
Inhibitory effect of atrial natriuretic factor on adenylate cyclase activity in adrenal cortical membranes
Author(s) -
Anand-Srivastava Madhu B.,
Genest Jacques,
Cantin Marc
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80259-3
Subject(s) - adenylate kinase , cyclase , medicine , endocrinology , forskolin , chemistry , hormone , stimulation , receptor , cholera toxin , biology
The effect of rat synthetic atrial natriuretic factor (ANF) on adenylate cyclase activity was studied in adrenal cortical membranes. Synthetic ANF (Arg 101‐Tyr 126) inhibited adenylate cyclase activity in a concentration‐dependent manner. The maximum inhibition observed was about 25% with an apparent K i of 5 x 10 −11 to 10 −10 M. Various hormones such as isoproterenol, dopamine, prostaglandin (PGE 1 ) and ACTH‐stimulated adenylate cyclase to various degrees and ANF inhibited the stimulatory effect of all these hormones to some degree but never abolished it. In addition, ANF was also able to inhibit the stimulation exerted by forskolin which activates adenylate cyclase by receptor‐independent mechanism. This is the first study demonstrating the inhibitory effect of ANF on adrenal cortical adenylate cyclase. From these results it can be suggested that the inhibition of adenylate cyclase may be one of the mechanisms through which ANF exerts the inhibitory effect on steroidogenesis stimulated by various hormones and agents.