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Search for a regulatory function of mitochondrial proteinases in yeast
Author(s) -
Viard I.,
Stahl A.J.C.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80243-x
Subject(s) - derepression , yeast , biochemistry , function (biology) , saccharomyces cerevisiae , mitochondrion , inner mitochondrial membrane , chemistry , psychological repression , phosphate , biology , microbiology and biotechnology , gene , gene expression
Proteolytic activity in yeast mitochondrial membranes and soluble fractions was studied using a labeled substrate: [ 14 C]dimethylcasein. Optimal activity for the two fractions was at pH 6 in phosphate buffer at 37°C. Activity was partially inhibited by adding 3 mmol/1 ATP. Activity was enhanced rapidly in both fractions when yeast cells were grown aerobically on a derepression medium and transfered on a repression medium in anaerobiosis. Mitochondrial proteinase mediation in the mitochondrial breackdown observed during transition from aerobical derepression to anaerobical repression is discussed.