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Thrombin induces the rapid formation of inositol bisphosphate and inositol trisphosphate in human platelets
Author(s) -
Siess Wolfgang,
Binder Helge
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80241-6
Subject(s) - inositol , phospholipase c , phosphatidylinositol , chemistry , inositol phosphate , inositol trisphosphate , second messenger system , thrombin , platelet , gq alpha subunit , ion chromatography , biochemistry , fast protein liquid chromatography , phospholipase , hydrolysis , chromatography , high performance liquid chromatography , biology , enzyme , receptor , signal transduction , g protein , immunology
Human platelets prelabeled with [ 3 H]inositol were exposed to thrombin. The aqueous soluble inositol phosphates were separated by anion exchange column chromatography, paper chromatography or high‐performance liquid chromatography, and identified by cochromatography with authentic standard substances. Thrombin immediately induces the rapid formation of inositol 1,4‐bisphosphate and inositol 1,4,5‐trisphosphate. Accumulation of inositol‐1‐monophosphate and inositol‐2‐monophosphate occurs later after a time lag of 10 sec. The results indicate that the phospholipase C induced polyphosphoinositide hydrolysis rather than the phosphatidylinositol hydrolysis is the triggering event for platelet activation, and support the concept of inositol 1,4,5‐trisphosphate as putative second messenger.