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Torus‐shaped particles formed due to intermolecular condensation of circular DNA upon interaction with synthetic tripeptide
Author(s) -
Vengerov Y.Y.,
Semenov T.E.,
Streltsov S.A.,
Makarov V.L.,
Khorlin A.A.,
Gursky G.V.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80236-2
Subject(s) - tripeptide , dna , intermolecular force , dna condensation , torus , condensation , chemistry , crystallography , pbr322 , biophysics , stereochemistry , plasmid , molecule , peptide , physics , biochemistry , biology , geometry , thermodynamics , organic chemistry , transfection , mathematics , gene
The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L‐Val‐L‐Val‐L‐Val‐NH‐NH‐Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus‐shaped particles. The torus parameter measurements offer the possibility to conclude that DNA condensation observed is of intermolecular nature. On the basis of the analysis of the structures corresponding to the early stages of DNA compaction the model for intermolecular condensation of circular DNA into torus‐shaped particles is proposed.