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Effect of polyethylene glycol on the kinetic behaviour of pyruvate kinase and other potentially regulatory liver enzymes
Author(s) -
Medina Ruth,
Aragón Juan J.,
Sols Alberto
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80235-0
Subject(s) - pyruvate kinase , enzyme , biochemistry , glycogen phosphorylase , polyethylene glycol , chemistry , pyruvate dehydrogenase kinase , pyruvate dehydrogenase complex , pyruvate decarboxylation , pyruvate dehydrogenase phosphatase , pyruvate dehydrogenase lipoamide kinase isozyme 1 , glucokinase , pkm2 , fructose , lactate dehydrogenase , kinase , glycolysis
Assay in the presence of 10% polyethylene glycol has been systematically used with potentially regulatory liver enzymes as an indirect way to induce aggregation of enzymes corresponding to that which could occur at their physiological concentrations. Pyruvate kinase L was markedly affected by polyethylene glycol, as was muscle phosphorylase a , while pyruvate kinase M as well as glucokinase, fructose‐1,6‐bisphosphatase and other liver enzymes examined were not affected.