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The vasoactive proteins in human urine
Author(s) -
Giorginis S.,
Dees C.,
Peterknecht S.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80230-1
Subject(s) - vasoactive , urine , vasoactive intestinal peptide , chemistry , chromatography , biochemistry , endocrinology , biology , neuropeptide , receptor
We have purified vasoactive polypetides from the urine of normotensive humans by gel filtration on Sephadex G150 superfine ionic exchange chromatography on DEAE‐cellulose and isoelectric focusing in poly‐ acrylamide gels using a pharmalyte pH range of 2.5–5.0. The purified polypetide fraction yielded four bands by isoelectric focusing with isoelectric points at pH 4.15, 4.05, 3.9 and 3.8. On dodecyl sulphate/polyacryl‐ amide gel electrophoresis (page) two bands appeared, one small band with a M r of 29 000 and one broad band ranging from M r 49 000 to M r 42 500. The polypetides lower the blood pressure of rabbits in a bioassay and cleave D‐valyl‐L‐leucyl‐L‐arginine‐4‐nitroanilide with a specific activity comparable to that of kallikrein.