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Effects of prostaglandin E 2 and cholera toxin on apical sodium uptake in thyroid epithelial cells: role of cAMP
Author(s) -
Gerard Corinne,
Verrier Bernard,
Mauchamp Jean
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80220-9
Subject(s) - cholera toxin , toxin , prostaglandin , chemistry , thyroid , sodium , prostaglandin e2 , endocrinology , medicine , biochemistry , biology , organic chemistry
When cultured in tissue culture polystyrene dishes, porcine thyroid cells formed polarized monolayers. Their apical pole was oriented towards the culture medium. Influx of sodium 22 (5 min) through the apical surface was partially inhibited by amiloride. Amiloride‐sensitive Na uptake was increased about 3‐fold by prostaglandin E 2 (1 μM, 30 min) and by cholera toxin (0.01 , 1 h). This increase, which was also obtained with forskolin and 8‐(4‐chlorophenylthio)adenosine‐3':5'‐monophosphate, cyclic (8‐chloro‐cAMP), is probably a consequence of the increase of the intracellular cAMP level by prostaglandin and cholera toxin.