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Phenylalanine ammonia‐lyase immobilized in semipermeable microcapsules for enzyme replacement in phenylketonuria
Author(s) -
Bourget Louis,
Chang Thomas M.S.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80219-2
Subject(s) - phenylalanine , chemistry , enzyme , phenylalanine ammonia lyase , immobilized enzyme , enzyme assay , ammonia , chromatography , biochemistry , semipermeable membrane , amino acid , membrane
Phenylalanine ammonia‐lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% ± 4% of the enzyme in free solution. The K m for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 ± 1.0. At the lower pH range, enzyme activity is higher for the immobilized enzyme. Daily oral administration of microencapsulated phenylalanine ammonia‐lyase to phenylketonuric rats decreased the systemic phenylalanine level by 35 ± 8% in 2 days ( P < 0.05) and by 75 ± 8% in 7 days ( P < 0.001).