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Isolation and characterization of neuropeptide Y from porcine intestine
Author(s) -
Tatemoto K.,
Siimesmaa S.,
Jörnvall H.,
Allen J.M.,
Polak J.M.,
Bloom S.R.,
Mutt V.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80216-7
Subject(s) - neuropeptide y receptor , peptide , neuropeptide , chemistry , radioimmunoassay , amino acid , biochemistry , protein primary structure , peptide sequence , microbiology and biotechnology , biology , gene , receptor
The isolation and primary structure of intestinal neuropeptide Y (NPY) is described. The peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for NPY. The amino acid sequence of this peptide is: Tyr‐Pro‐Ser‐Lys‐Pro‐Asp‐Asn‐Pro‐Gly‐Glu‐Asp‐Ala‐Pro‐Ala‐Glu‐Asp‐LeuAla‐Arg‐Tyr‐Tyr‐Ser‐Ala‐Leu‐Arg‐His‐Tyr‐Ile‐Asn‐Leu‐Ile‐Thr‐Arg‐Gln‐Arg‐Tyr‐NH 2 . This indicates that the structure of intestinal NPY is identical to the NPY of brain origin.