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Site‐specific restriction endonuclease from the filamentous cyanobacterium Nostoc sp. MAC PCC 8009
Author(s) -
Lau Reginald H.,
Visentin Louis P.,
Martin Stan M.,
Hofman Jason D.,
Doolittle W.Ford
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80206-4
Subject(s) - isoschizomer , restriction enzyme , endonuclease , nostoc , lysis , chemistry , deoxyribonucleases , restriction fragment , cyanobacteria , dna , biochemistry , enzyme , microbiology and biotechnology , biology , genetics , bacteria , gene , gene expression , dna methylation
We report here the presence of a type‐II restriction endonuclease in the filamentous cyanobacterium Nostoc sp. MAC PCC 8009. This restriction enzyme, NSp MAC I, is the first reported isoschizomer of Bgl II and is very readily purified from non‐specific deoxyribonuclease activity in the crude lysate by one round of phosphocellulose column chromatography.