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Monoclonal antibodies localize the exchangeable GTP‐binding site in β‐ and not α‐tubulins
Author(s) -
Hesse Juliane,
Maruta Hiroshi,
Isenberg Gerhard
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80198-8
Subject(s) - monoclonal antibody , gtp' , binding site , chemistry , antibody , microbiology and biotechnology , biology , virology , biochemistry , genetics , enzyme
A combination of several methods was used to localize the exchangeable GTP‐binding site in the α‐/β‐tubulin heterodimer: (i) direct photoaffinity labeling with [α‐ 32 P]GTP, (ii) specific labeling of α‐ and β‐tubulin by tyrosylation and phosphorylation, respectively, and (iii) immunoprecipitation with specific monoclonal antibodies. Direct evidence was obtained that GTP binds exclusively to β‐ and not α‐tubulins.