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Picosecond time‐resolved fluorescence spectra of photosystem I and II in Chlorella pyrenoidosa
Author(s) -
Yamazaki I.,
Mimuro M.,
Tamai N.,
Yamazaki T.,
Fujita Y.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80192-7
Subject(s) - p700 , picosecond , fluorescence , chlorella pyrenoidosa , photochemistry , photosystem i , photosystem ii , photosynthetic reaction centre , chemistry , analytical chemistry (journal) , chlorophyll fluorescence , spectral line , electron transfer , photosynthesis , chlorella , optics , physics , laser , biology , algae , botany , chromatography , biochemistry , astronomy
Picosecond time‐resolved fluorescence spectra emitted from intact cells of the green alga Chlorella pyrenoidosa have been measured by means of a new detection technique using a microchannel‐plate photomultiplier. A fluorescence band (F700) was observed at 690–730 nm in the initial time region (0–180 ps), in addition to the well‐known spectrum (F685) of photosystem II (PS II)‐chlorophyll a (Chl a ) with a peak at 685 nm. F700 decays rapidly with lifetime of 104 ps, while F685 decays much more slowly in bi‐exponential form with lifetimes of 0.64 and 1.7 ns. Appearance of F700 is independent of closure of the reaction center II (RC II). F700 is thus assigned to the fluorescence from PS I‐Chl a , whose decay is governed by a fast energy transfer process from the antenna Chl a of PS I to P700 of RC I.