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A simple and rapid method for sequencing DNA
Author(s) -
Chuvpilo S.A.,
Kravchenko V.V.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80185-x
Subject(s) - dna , cleavage (geology) , chemistry , combinatorial chemistry , sequence (biology) , dna sequencing , chromatography , computational biology , computer science , microbiology and biotechnology , biology , biochemistry , paleontology , fracture (geology)
A simplified technique for DNA sequence analysis has been developed, based on modification of a previous method [(1980) Methods Enzymol. 65, 499‐560]. It employs an adsorptive immobilization of terminally labelled DNA on DEAE paper followed by G, A + G, C + T and C specific modification and cleavage reactions. This solid‐phase technique is faster and more convenient than the original method. The efficiency is comparable. The total processing time taken to produce cleaved fragments loaded on a gel is less than 2h.