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Presence of tropomyosin in adrenal chromaffin cells and its association with chromaffin granule membranes
Author(s) -
Burgoyne Robert D.,
Norman Kathyrn-Marie
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80183-6
Subject(s) - chromaffin cell , tropomyosin , adrenal medulla , biology , immunoperoxidase , microbiology and biotechnology , biochemistry , membrane , granule (geology) , actin , antibody , catecholamine , endocrinology , monoclonal antibody , immunology , paleontology
The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti‐tropomyosin antibody. In immunoblotting the antibody labelled polypeptides of M r 39000, 36500 and 31000 in 1 × g supernatants and heat‐stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39‐kDa and 31‐kDa polypeptides comigrated with polypeptides labelled by anti‐tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39‐kDa polypeptide that was labelled by anti‐tropomyosin.