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Inhibition of the activity of restriction endonucleases by spermidine and spermine
Author(s) -
Kuosmanen Malla,
Pösö Hannu
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80181-2
Subject(s) - spermidine , spermine , restriction enzyme , dna , biochemistry , chemistry , recombinant dna , ecori , enzyme , polyamine , plasmid , microbiology and biotechnology , in vitro , biology , gene
Physiological concentrations (0.5–2.0 mM) of spermidine and spermine were observed to inhibit the digestion in vitro of plasmid pJDB 207 by the restriction endonucleases Bam HI (EC 3.1.23.6), Eco RI (EC 3.2.23.13), Hin dIII (EC 3.1.23.20), Hpa I (EC 3.1.23.23) and Pst I (EC 3.1.23.31). The polyamines protected all the tested restriction sequences of DNA, since the activity of all endonucleases used was strongly inhibited. These results show the need for caution when using polyamines as experimental tools for recombinant DNA chemistry.