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Multiple relationships between rate of oxidative phosphorylation and Δ µ̃ H in rat liver mitochondria
Author(s) -
Zoratti Mario,
Petronilli Valeria
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80168-x
Subject(s) - malonate , oxidative phosphorylation , mitochondrion , electrochemical gradient , oxidizing agent , biochemistry , chemistry , respiratory chain , atp synthase , respiration , biology , biophysics , membrane , anatomy , enzyme , organic chemistry
The relationship between rate of ATP synthesis and transmembrane electrochemical proton gradient has been determined in rat liver mitochondria oxidizing succinate, using the respiratory inhibitor malonate or the uncoupler FCCP to decrease Δ µ̃ H progressively. As previously reported [(1982) Eur. J. Biochem. 126, 443‐451] two different relationships are obtained depending on the method used. Evidence is presented that this result is not due to underestimation of the Δ µ̃ H maintained by fast‐respiring mitochondria, as recently suggested [(1985) FEBS Lett. 181, 323‐327].