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Tenacious binding of lipids to vimentin during its isolation and purification from Ehrlich ascites tumor cells
Author(s) -
Traub Peter,
Perides Georgios,
Scherbarth Annemarie,
Traub Ulrike
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80155-1
Subject(s) - chemistry , chromatography , vimentin , urea , extraction (chemistry) , chloroform , ehrlich ascites , biochemistry , sepharose , thin layer chromatography , column chromatography , tumor cells , biology , enzyme , immunohistochemistry , cancer research , immunology
Vimentin enriched in cytoskeletal frameworks by Triton X‐100 extraction of Ehrlich ascites tumor cells and purified from a low ionic strength extract of the cell residues by (NH 4 ) 2 SO 4 precipitation and DEAE‐Sepharose and ssDNA‐cellulose chromatography in the presence of 6 M urea was highly contaminated with lipids. Thin‐layer chromatography of a chloroform‐methanol extract of the purified protein revealed, besides small amounts of phospholipids, the presence of large quantities of neutral lipids.