Premium
Azidocytidine is incorporated into RNA of 3T6 mouse fibroblasts
Author(s) -
Åkerblom Lennart
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80151-4
Subject(s) - cytidine , ribonucleotide reductase , rna , ribonucleotide , dna , dna synthesis , chemistry , nucleoside , enzyme , biochemistry , microbiology and biotechnology , biology , nucleotide , gene , protein subunit
Earlier work has shown that azidocytidine inhibits the growth and DNA synthesis of 3T6 mouse fibroblasts by inactivation of the enzyme ribonucleotide reductase. RNA synthesis, as measured by incorporation of [ 3 H]cytidine was not affected. Here I show that azidocytidine is incorporated into RNA, but not into DNA. Incorporation of the analogue into RNA may under special circumstances contribute to the biological effect of the nucleoside.