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Synthesis of dinucleoside tetraphosphates by RNA polymerase B (II) from calf thymus
Author(s) -
Hinrichsen Angelika I.,
Ortner Irmgard M.,
Hartmann Guido R.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80150-2
Subject(s) - polymerase , microbiology and biotechnology , chemistry , rna polymerase , rna , biology , biochemistry , dna , gene
Highly purified RNA polymerase B (II) from calf thymus catalyses the synthesis of dinucleoside tetraphosphates from ribonucleoside triphosphates in the absence of an oligonucleotide primer or additional protein factors. The reaction requires a DNA template and bivalent cations such as Mn 2+ or Mg 2+ . It is strongly inhibited by heparin and high concentrations of α‐amanitin but not by rifampicin. On a given template various dinucleoside tetraphosphates of different sequence are formed although the yield depends on the nature of the template.