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6‐Phosphogluconolactonase from Zymomonas mobilis
Author(s) -
Scopes R.K.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80147-2
Subject(s) - zymomonas mobilis , size exclusion chromatography , chemistry , chromatography , enzyme , monomer , biochemistry , ethanol , organic chemistry , ethanol fuel , polymer
The enzyme 6‐phosphogluconolactonase (EC 3.1.1.31) is present at high levels in Zymomonas mobilis cells. A simple procedure for its isolation involving dye‐ligand chromatography and gel filtration has resulted in a 500‐fold purification with high recovery. The purified enzyme is a monomer of 26 kDa, and has a high catalytic efficiency with of 9 × 10 7 M −1 s −1 at 25° C. Two assay procedures for the enzyme are compared, and a simple method of obtaining a solution of 6‐phosphoglucono‐δ‐lactone relatively free of other metabolites is presented.