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Resistance to alloxan of tumoral insulin‐producing cells
Author(s) -
Sener Abdullah,
Malaisse Willy J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80140-x
Subject(s) - alloxan , intracellular , extracellular , pancreatic islets , endocrinology , medicine , insulin , cytotoxic t cell , chemistry , islet , biology , biochemistry , diabetes mellitus , in vitro
Rat pancreatic islets and insulin‐producing cells of the RINm5F line were incubated for 5 min at 7 or 23°C in media containing 3 H 2 O and either L‐[1‐ 14 C]glucose or [2‐ 14 C]alloxan. In the islets the intracellular distribution space of [2‐ 14 C]alloxan represented, at 7 and 23°C respectively, 11.4 ± 1.0 and 25.5 ± 2.3% of the intracellular 3 H 2 O space. In the RINm5F cells, the distribution space of [2‐ 14 C]alloxan failed to be affected by the ambient temperature and represented, after correction for extracellular contamination, no more than 5.2 ± 0.5% of the intracellular 3 H 2 O space. Preincubation for 30 min at 7°C in the presence of alloxan (10 mM) failed to affect subsequent D‐[U‐ 14 C]glucose oxidation in the tumoral cells, whilst causing a 70% inhibition of glucose oxidation in the islets. It is proposed that RINmSF cells are resistant to the cytotoxic action of alloxan, this being attributable, in part at least, to poor uptake of the diabetogenic agent.