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Monensin‐dependent and ‐independent mechanisms of cell‐matrix adhesion
Author(s) -
Niven Veronica M.,
Aplin John D.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80138-1
Subject(s) - monensin , intracellular , adhesion , chemistry , cell , cell adhesion , microbiology and biotechnology , cell culture , biophysics , matrix (chemical analysis) , biochemistry , biology , chromatography , genetics , organic chemistry
Attachment and spreading of human FL cells on a subcellular matrix (SCM) preparation made by treating confluent cell monolayers with deoxycholate are insensitive to the presence of monensin. However, if the cell suspension is surface‐iodinated prior to adhesion using the LPO/H 2 O 2 system, cell spreading on SCM is inhibited by 1 μM monensin. The suggested interpretation is that cell surface components required for cell spreading on SCM are inactivated by iodination and need replacement from intracellular reserves by a monensin‐sensitive pathway. This pathway is not required in the absence of iodination when sufficient surface components (or a monensin‐independent pathway of surface expression) are available. Support for this interpretation is obtained by means of double‐iodination experiments in which surface‐labelled cells adhere and spread, are detached and labelled a second time and then allowed to adhere again to SCM. Cell spreading in the second case is inhibited by ~ 80%, suggesting that both previously expressed and newly recruited receptors are inactivated.