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Spin label studies of the essential sulfhydryl group environment in chicken liver fructose‐1,6‐bisphosphatase
Author(s) -
Zeidan Henry,
Han Peter,
Johnson Joe
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80128-9
Subject(s) - fructose 1,6 bisphosphatase , iodoacetamide , chemistry , spin label , fructose , enzyme , nitroxide mediated radical polymerization , biochemistry , stereochemistry , cysteine , organic chemistry , polymer , polymerization , radical polymerization , membrane
The local environment of the essential sulfhydryl groups in chicken liver fructose‐1,6‐bisphosphatase has been investigated by ESR techniques using a series of iodoacetamide spin labels, varying in chain length between the iodoacetate and nitroxide free radical group. The ESR spectrum of spin‐labeled chicken liver fructose‐1,6‐bisphosphatase showed that the sites of labeling were highly immobilized when the enzyme was chemically modified by spin label iodoacetate, suggesting that the sulfhydryl groups of the protein are in a small, confined environment. From the change in the ESR spectra of these nitroxides as a function of chain length, we conclude that the sulfhydryl group is located in a cleft approx. 10.5 Å in depth.