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Molecular forms of glucagon‐like peptides in man
Author(s) -
George S.K.,
Uttenthal L.O.,
Ghiglione M.,
Bloom S.R.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80124-1
Subject(s) - proglucagon , radioimmunoassay , glucagon , pancreas , cleavage (geology) , chemistry , biochemistry , glucagon like peptide 1 , small intestine , glucagon like peptide 2 , glucagonoma , peptide , microbiology and biotechnology , biology , endocrinology , hormone , paleontology , fracture (geology) , type 2 diabetes , diabetes mellitus
Molecular forms of the glucagon‐like peptides (GLP) encoded by the human preproglucagon gene were analysed by chromatography combined with specific radioimmunoassays to the synthetic peptides. Whereas extracts of human pancreas and a glucagonoma contained a large proglucagon cleavage product possessing both GLP‐1 and GLP‐2 immunoreactivities, extracts of human intestine contained products corresponding to free GLP‐1 and a small amount ofchromatographically distinct GLP‐2 immunoreactivity. It is concluded that post‐translational processing of proglucagon differs in pancreas and intestine, so that the C‐terminal portion of the molecule is cleaved to liberate free GLP‐1 in the intestine. Further processing or degradation results in loss especially of GLP‐2 immunoreactivity.