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Rapid isolation of animal mitochondrial DNA by alkaline extraction
Author(s) -
Palva Tuula K.,
Palva E.Tapio
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80122-8
Subject(s) - mitochondrial dna , nucleic acid , dna , dna extraction , cloning (programming) , isolation (microbiology) , microbiology and biotechnology , centrifugation , biochemistry , chemistry , biology , extraction (chemistry) , nuclear dna , chromatography , polymerase chain reaction , gene , computer science , programming language
A simple technique for rapid isolation of mitochondrial DNA (mt DNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513‐1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to that obtained by standard techniques. This DNA is sufficiently pure for restriction analysis and cloning of mtDNA fragments.