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Enhancement of O 2 − generation and tumoricidal activity of murine macrophages by a monosaccharide precursor of Escherichia coli lipid A
Author(s) -
Amano Fumio,
Nishijima Masahiro,
Akagawa Kiyoko,
Akamatsu Yuzuru
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80121-6
Subject(s) - lipid a , lipopolysaccharide , escherichia coli , macrophage , lipid metabolism , monosaccharide , chemistry , biochemistry , cytotoxicity , glycolipid , cell culture , phosphate , cytotoxic t cell , biology , microbiology and biotechnology , immunology , gene , in vitro , genetics
The effects of a monosaccharide precursor of Escherichia coli lipid A (lipid X) on murine macrophages were studied. Lipid X is a diacylglucosamine 1‐phosphate bearing β‐hydroxymyristoyl groups at positions 2 and 3. Lipid X, as well as lipopolysaccharide and lipid A, enhanced O 2 − generation in mouse peritoneal macro‐phages and a macrophage‐like cell line, J774.1, and further induced the tumor‐cytotoxic activity of peritoneal macrophages. Elimination of a 1‐phosphate or 3‐ O ‐β‐hydroxymyristoyl group from lipid X completely abolished these effects, suggesting that both 1‐phosphate and 3‐ O ‐β‐hydroxymyristoyl groups are essential for the elevated O 2 − generation and induction of turmoricidal activity due to lipid X.