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Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP
Author(s) -
Masturzo P.,
Salmona M.,
Nordstrom O.,
Consolo S.,
Ladinsky H.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80106-x
Subject(s) - oxotremorine , muscarinic acetylcholine receptor , microviscosity , endocrinology , medicine , pirenzepine , stimulation , chemistry , muscarinic agonist , membrane fluidity , agonist , muscarinic acetylcholine receptor m1 , receptor , membrane , biophysics , biology , biochemistry
The muscarinic agonist oxotremorine produced a linear dose‐dependent increase in membrane fluidity of intact and viable human lymphocytes in vitro. This effect proved to be receptor‐mediated because preincubation with 10 −5 M atropine shifted the dose‐response curve one order of magnitude rightward. Pirenzepine preincubation did not affect membrane fluidity variation. A cGMP increase was also found after oxotremorine treatment. The results are discussed in terms of possible modulation ofguanyl cyclase and adenyl cyclase through membrane fluidity variations.