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The H + /O ratio of proton translocation linked to the oxidation of succinate by mitochondria
Author(s) -
Lehninger Albert L.,
Reynafarje Baltazar,
Hendler Richard W.,
Shrager Richard I.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80102-2
Subject(s) - stoichiometry , mitochondrion , chemistry , chromosomal translocation , proton , crystallography , stereochemistry , biophysics , physics , biochemistry , biology , nuclear physics , gene
Costa, L.E., Reynafarje, B. and Lehninger, A.L. [(1984) J. Biol. Chem. 259, 4802‐4811] have reported ‘second‐generation’ measurements of the H + /O ratio approaching 8.0 for vectorial H + translocation coupled to succinate oxidation by rat liver mitochondria. In a Commentary in this Journal [Krab, K., Soos, J. and Wikström, M. (1984) FEBS Lett. 178, 187‐192] it was concluded that the measurements of Costa et al. significantly overestimated the true H + /O stoichiometry. It is shown here that the mathematical simulation on which Krab et al. based this claim is faulty and that data reported by Costa et al. had already excluded the criticism advanced by Krab et al. Also reported are new data, obtained under conditions in which the arguments of Krab et al. are irrelevant, which confirm that the H + /O ratio for succinate oxidation extrapolated to level flow is close to 8.