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40 S subunits from rat liver ribosomes contain two codon‐dependent sites for transfer RNA
Author(s) -
Semenkov Yu.P.,
Kirillov S.V.,
Stahl J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80088-0
Subject(s) - transfer rna , ribosome , chemistry , biochemistry , rna , protein quaternary structure , protein subunit , biology , stereochemistry , gene
40 S subunits from rat liver ribosomes are able to bind, after heat activation, two molecules of either Phe‐tRNA phe , Ac‐Phe‐tRNA phe or deacylated tRNA phe . Addition of 60 S subunits to the quaternary complex 40 S·poly(U)·(Phe‐tRNA phe ) 2 results in quantitative formation of (Phe) 2 ‐tRNA phe . This indicates that the two binding sites for tRNA on 40 S subunits should be considered as the constituent of P and A sites of 80 S ribosomes.