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The determination of intracellular water space by NMR
Author(s) -
Shinar Hadassah,
Navon Gil
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80082-x
Subject(s) - intracellular , chemistry , nuclear magnetic resonance spectroscopy , imidazole , analytical chemistry (journal) , cobalt , nuclear magnetic resonance , chromatography , inorganic chemistry , biochemistry , stereochemistry , physics
A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59 Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN) 6 3− or Co(imidazole) 6 3+ and the 2 H or 1 H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured