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Stimulation of protein accumulation in HeLa cells by inhibitors of DNA replication
Author(s) -
Menozzi F.D.,
Hanotte O.,
Miller A.O.A.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80077-6
Subject(s) - aphidicolin , ferritin , dna replication , dna synthesis , hela , microbiology and biotechnology , dna , thymidine , chemistry , dna polymerase , rna , stimulation , protein biosynthesis , butyrate , biology , incubation , biochemistry , gene , in vitro , fermentation , neuroscience
Incubation of HeLa cells for 24 h with either hydroxyurea (HU), aphidicolin (APHI), thymidine (T) or butyrate (BU), substances used to inhibit replication and accumulate cells at the G 1 /S interphase, followed by the elimination of the inhibitor and the addition of iron to the growth medium, results in an immediate (HU, APHI, T) or slightly delayed (BU) increased accumulation (18–24‐fold higher than the basal level) of ferritin. Under the same experimental circumstances, 5‐azacytidine is without effect. As a result of the action of these inhibitors on the structure of DNA, it is proposed that ferritin genes remain accessible to RNA polymerase allowing the accumulation in the cytoplasm of mature ferritin mRNA ready to be mobilized by iron for the production of ferritin molecules.