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Protein turnover in the cytoplasmic transport system within an insect ovary ‐ a clue to the mechanism of microtubule‐associated transport
Author(s) -
Stebbings H.,
Sharma K.,
Hunt Cherryl
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80071-5
Subject(s) - microtubule , cytoplasm , mechanism (biology) , microbiology and biotechnology , insect , transport protein , biophysics , ovary , chemistry , biology , botany , physics , genetics , quantum mechanics
The movement of radioactively labelled polypeptides into the microtubule‐associated transport channels in the ovaries of a hemipteran insect has been analysed using SDS‐polyacrylamide slab gel electrophoresis and fluorography. The patterns of label suggest that the microtubules which pack the transport channels form a relatively static cytoskeleton while other components move independently from them along the channels. As well as illustrating the functional organisation of microtubule‐associated transport in this system our studies of labelled proteins have also provided clues as to the mechanism of transport itself.