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Lipid domain in cancer cell plasma membrane shown by 1 H NMR to be similar to a lipoprotein
Author(s) -
Williams Philip G.,
Helmer Megan A.,
Wright Lesley C.,
Dyne Marlen,
Fox Richard M.,
Holmes Kerry T.,
May George L.,
Mountford Carolyn E.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80064-8
Subject(s) - very low density lipoprotein , chemistry , lipoprotein , membrane , cancer cell , triglyceride , cholesterol , biochemistry , cancer , biophysics , medicine , biology
Human blood lipoproteins have been characterised by 1 H NMR methods and chemical analysis, and comparisons made with the properties of the triglyceride‐rich plasma membrane domain found in cancer cells. By means of selective and non‐selective T 1 experiments, the lipids in HDL and LDL are shown to be in diffusive exchange. In contrast, the lipids ofchylomicra and VLDL do not exhibit lipid diffusion, and therefore resemble the neutral lipids of cancer cell plasma membranes. 2D scalar correlated NMR (COSY) spectra of cancer cells or solid tumours are similar to those obtained from VLDL and LDL. The long T 2 relaxation value observed for neutral lipid methylenes in metastatic cancer cells (>00 ms) was not observed for any of the 4 lipoproteins studied. None of the lipoprotein classes gave a T 2 longer than 250 ms.