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Identification of microtubule‐associated proteins (MAPs) in Xenopus oocyte
Author(s) -
Jessus C.,
Thibier C.,
Ozon R.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80059-4
Subject(s) - xenopus , microtubule , tubulin , oocyte , microbiology and biotechnology , microtubule associated protein , biology , prophase , chemistry , biochemistry , embryo , meiosis , gene
Microtubules were isolated from prophase‐blocked oocytes of Xenopus laevis with the use of the anti‐tumor drug taxol. In addition to tubulin, 5 microtubule‐associated proteins (MAPs) were characterized. Among them, 2 high molecular mass proteins (200–300 kDa) are phosphorylated in ovo. The oocyte MAP extract promotes the assembly of rat brain 6 S purified tubulin.