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Rotating frame nuclear overhauser effect: a practical tool for the 1 H NMR study of peptides in solution
Author(s) -
Marion Dominique
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80051-x
Subject(s) - nuclear overhauser effect , two dimensional nuclear magnetic resonance spectroscopy , chemistry , nuclear magnetic resonance spectroscopy , spectroscopy , nuclear magnetic resonance , analytical chemistry (journal) , stereochemistry , physics , chromatography , quantum mechanics
A new 2D NMR experiment, the rotating frame nuclear Overhauser enhancement (nOe) spectroscopy (ROESY) was recently proposed as an alternative to NOESY for the study of intermediate molecules. Its practical use is here tested on bacillomycin D, a bacterial cyclic lipopeptide. This sequential resonance assignment, based on nOe connectivities at room temperature, shows that the high viscosity which is generally required for conventional nOe measurement is no longer a restriction for choosing a suitable solvent. In comparison to low‐temperature NOESY, short internuclear connectivities can be safely demonstrated because of no spin diffusion, for sequential assignment ofpeptide resonances.