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Structural differences in the cell binding region of human fibronectin molecules isolated from cultured normal and tumor‐derived human cells
Author(s) -
Borsi Laura,
Allemanni Giorgio,
Castellani Patrizia,
Rosellini Claudio,
Di Vinci Angela,
Zardi Luciano
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80045-4
Subject(s) - fibronectin , fibronectins , chemistry , molecule , microbiology and biotechnology , cell , cell culture , biophysics , biochemistry , biology , genetics , organic chemistry
Fibronectins isolated from human plasma (pFN) and from the conditioned media of normal (N‐cFN) and tumor (T‐cFN) human cells were compared by cathepsin D digestion followed by immunostaining of released fragments with the monoclonal antibody 3E3, specific for the cell binding site. Two different staining patterns were obtained, one specific for pFN and N‐cFN, the second common to fibronectins from the 3 different kinds of tumors studied. This indicates structural differences between N‐cFN and T‐cFN in the cell binding region of the fibronectin molecule.