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A restriction endonuclease Sua I from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius
Author(s) -
Prangishvili D.A.,
Vashakidze R.P.,
Chelidze M.G.,
Gabriadze I.Yu.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80042-9
Subject(s) - sulfolobus acidocaldarius , restriction enzyme , isoschizomer , biology , sulfolobus , biochemistry , enzyme , dna , nucleotide , endonuclease , archaea , gene , gene expression , dna methylation
A type II restriction endonuclease ( Sua I) has been isolated from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius . The enzyme is an isoschizomer of Bsp RI. It does not cut S. acidocaldarius DNA, as the recognition sequence GGCC in this DNA contains modified nucleotide(s). The enzyme is most active at 60–70°C and is highly thermostable.