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Structural analysis of a new GC‐specific insertion element IS186
Author(s) -
Chong Pele,
Hui Ivy,
Loo Tip,
Gillam Shirley
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80040-5
Subject(s) - open reading frame , insertion sequence , stop codon , inverted repeat , genetics , nucleotide , base pair , biology , sequence (biology) , start codon , nucleic acid sequence , escherichia coli , amino acid , chemistry , peptide sequence , gene , transposable element , genome
A new insertion sequence, IS186, has been identified from Escherichia coli and sequenced. It contains 1336 base pairs with a terminal inverted repeat of 22 nucleotides. A long open reading frame, from an ATG codon at position 55 to a TAG termination codon at 1327, could code for a polypeptide of 424 amino acids. This element recognizes GC‐rich regions as target sites for insertion.