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Oligomer distribution of depolymerase digests: comparison of theory and experiment
Author(s) -
Mazur A.K.,
Nazarova N.I.,
Elyakova L.A.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80039-9
Subject(s) - oligomer , hydrolysis , chemistry , chromatography , distribution (mathematics) , product distribution , exponential function , enzyme , enzymatic hydrolysis , exponential distribution , organic chemistry , mathematics , mathematical analysis , statistics , catalysis
Enzyme hydrolysis of linear β‐1,3‐glucan was carried out, with p ‐nitrophenylglucoside (in a high concentration) involved in simultaneous transglucosylation reaction. The p ‐nitrophenyloligosides were next separated by gel chromatography and the product distribution shape was studied. In good agreement with earlier theoretical predictions the distribution shape was close to an exponential one for oligomer chain length exceeding the size of the enzyme‐binding region.