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Ca 2+ ‐independent secretion is dependent on cytoplasmic ATP in human platelets
Author(s) -
Sanchez Ana
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80025-9
Subject(s) - secretion , diacylglycerol kinase , cytoplasm , platelet , thrombin , microbiology and biotechnology , adenosine triphosphate , chemistry , secretory protein , protein kinase c , biochemistry , biophysics , biology , kinase , immunology
The secretion evoked in human platelets by physiological agonists is an energy‐requiring process that depends on the metabolic ATP pool. Diacylglycerol, phorbol ester and collagen evoke a secretory response, which is not associated with an increase of cell Ca 2+ levels, and is attributed to activation of protein kinase C [(1983) Nature 305, 317‐319]. The secretion evoked by these agonists decreased along with cytoplasmic ATP depletion in the same way that the thrombin‐induced secretion did. The secretory response was restored by raising again the cytoplasmic ATP levels. These results support the idea that the secretory response takes place by the physiological ATP‐dependent mechanisms rather than by membrane perturbations in these instances.