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Evidence that a chlorophyll a ' dimer constitutes the photochemical reaction centre 1 (P700) in photosynthetic apparatus
Author(s) -
Watanabe Tadashi,
Kobayashi Masami,
Hongu Akinori,
Nakazato Masataka,
Hiyama Tetsuo,
Murata Norio
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80019-3
Subject(s) - p700 , p680 , dimer , photochemistry , chemistry , photosystem i , chlorophyll , chlorophyll a , light harvesting complexes of green plants , photosynthesis , photosynthetic reaction centre , photosystem ii , electron transfer , organic chemistry , biochemistry
Spinach chloroplasts and their various fractions enriched in either photochemical reaction centre 1 (P700) or reaction centre 2 (P680) have been analyzed for pigment composition by high‐performance liquid chromatography (HPLC). The results clearly establish a stoichiometry of chlorophyll (Chl) a' /P700 = 2, where Chl a ' represents the C 10 epimer of Chl a . This is the first demonstration of the association of Chl a' with any functional site in vivo. It is further shown that a Chl a ' dimer prepared in vitro reproduces fairly well the redox and spectroscopic behaviour of P700. These findings provide strong evidence that a Chl a dimer constitutes P700.