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Lymphocyte glutathione status in relation to their Con A proliferative response
Author(s) -
Lacombe Philippe,
Kraus Laurence,
Fay Michel,
Pocidalo Jean-Jacques
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80013-2
Subject(s) - glutathione , immunology , lymphocyte , chemistry , relation (database) , biology , biochemistry , computer science , enzyme , database
We studied the intracellular total, oxidized and reduced glutathione levels in thymus and spleen rat lymphocytes cultured with or without Con A and 2‐mercaptoethanol (2‐ME). After 48 h culture, the total glutathione level decreased and the oxidized glutathione level increased in the two types of unstimulated and stimulated cells. In the presence of 2‐ME, the tritiated thymidine incorporation increased in splenocytes but not in thymocytes; on the other hand, the two types of stimulated cells increased their total and oxidized glutathione content. The enhancement of the GSSG/GSH + GSSG ratio, irrespective of culture conditions, indicates a severely disturbed redox state of the cells. 2‐ME acts on the glutathione synthesis of stimulated lymphocytes but is unable to maintain a normal redox state of these cells.