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Isolation and partial characterization of gamone 1 of Euplotes octocarinatus
Author(s) -
Weischer Annette,
Freiburg Manfred,
Heckmann Klaus
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80003-x
Subject(s) - ciliate , biology , isolation (microbiology) , mating type , microbiology and biotechnology , genetics , gene
A polypeptide, termed gamone 1, was isolated and purified to homogeneity from culture filtrates of mating type VII of the freshwater ciliate Euplotes octocarinatus . The gamone induces intraclonal conjugation in cells of certain other mating types. The isolation and purification of the gamone was carried out by a combination of two chromatographic steps. The purified gamone was found to be still effective in a concentration of approx. 10 −15 M.